Abstract

Research Article

Study on the Molecular Mechanism of Antioxidant Health Effect of Tuna Dark Meat Enzymatic Polypeptides

Zhang Diya, Dong Xin* and Guo Chunyu

Published: 21 March, 2024 | Volume 8 - Issue 1 | Pages: 011-015

In order to study the molecular mechanism of the antioxidant effect of enzymatically hydrolyzed tuna dark meat peptides, this article uses alkaline protease to enzymatically hydrolyze tuna dark meat, and at the same time performs peptide sequencing using matrix-assisted laser dissociation time-of-flight mass spectrometry (MALDI TOF/TOF). Discovery Studio (DS) performed molecular docking. Finally, the antioxidant effect was verified through DPPH clearance experiments. The results show that the dominant peptide sequences in the tuna dark meat hydrolyzed polypeptides  are LAPGQ, GGGDPI, and PLRLP; through molecular simulation methods (Discover Studio, DS), the potential target of the above-mentioned enzymatic polypeptides was screened out to be Keap1, thus predicting antioxidant activity. It provides theoretical support for further research on enzymatic peptides. Through DPPH clearance experiments, it was found that both the enzymatic hydrolysate and LAPGQ, GGGDPI, and PLRLP have antioxidant activity, confirming their effects.

Read Full Article HTML DOI: 10.29328/journal.ascr.1001078 Cite this Article Read Full Article PDF

Keywords:

Tuna by-products; Enzymatic peptides; Molecular docking

References

  1. Herpandi NH, Rosma A, Nadiah WAW. The tuna fishing industry: a new outlook on fish protein hydrolysates. Comprehensive Reviews in Food Science & Food Safety. 2011; 10(4): 195-207.
  2. Sohn JH, Ohshima T. Control of lipid oxidation and meat color deterioration in skipjack tuna muscle during ice storage. Fisheries Science. 2010; 76(4): 703-710.
  3. Su Y. Analysis of Nutrient Composition and Study on Anti fatigue Activity of Muscle Protease Hydrolysates of Three Tuna Species from the South China Sea. Guangdong: Guangdong Ocean University, 2015.
  4. Su Y, Zhang C H, Cao WH. Analysis and Evaluation of Nutritional Composition of Three Kinds of Tuna Common Meat and Dark Meat Produced in the South China Sea [J] Journal of Guangdong Ocean University. 2015; 35(3):87-93.
  5. Je JY, Qian ZJ, Byun HG. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochemistry. 2007; 42(5): 840-846.
  6. Je JY, Lee KH, Mi HL. Antioxidant and antihypertensive protein hydrolysates produced from tuna liver by enzymatic hydrolysis. Food Research International. 2009; 42(9): 1266-1272.
  7. Liu JH, Wang B, Kuai P. Enzymatic hydrolysis process of dark tuna meat and nutritional value evaluation of its hydrolysates. Food Science. 2014; 35(20): 1-5.
  8. Han J, Tang S, Li Y. In silico analysis and in vivo tests of the tuna dark muscle hydrolysate anti-oxidation effect. Rsc Advances. 2018; 8(25): 14109-14119.
  9. Xin J M, Wang P, Li S H. A Study on the Technology of Compound Enzymatic Hydrolysis of Broken Tuna Meat. The Food Industry. 2011(9); 25-28.
  10. Qian ZJ, Je JY, Kim SK. Antihypertensive effect of angiotensin i converting enzyme-inhibitory peptide from hydrolysates of Bigeye tuna dark muscle, Thunnus obesus. J Agric Food Chem. 2007 Oct 17;55(21):8398-403. doi: 10.1021/jf0710635. Epub 2007 Sep 26. PMID: 17894458.
  11. Hsu KC. Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product. Food Chemistry. 2010; 122(1): 42-48.
  12. Park K, Cho AE. Using reverse docking to identify potential targets for ginsenosides. Journal of Ginseng Research.
  13. Grienke U, Kaserer T, Pfluger F, Mair CE, Langer T, Schuster D, Rollinger JM. Accessing biological actions of Ganoderma secondary metabolites by in silico profiling. Phytochemistry. 2015 Jun;114:114-24. doi: 10.1016/j.phytochem.2014.10.010. Epub 2014 Nov 6. PMID: 25457486; PMCID: PMC4948669.
  14. Xie CL, Chong SY, Cao GP, In silico investigation of action mechanism of four novel angiotensin-I converting enzyme inhibitory peptides modified with Trp. Journal of Functional Foods. 2015; 17: 632-639.
  15. Tu M, Wang C, Chen C, Zhang R, Liu H, Lu W, Jiang L, Du M. Identification of a novel ACE-inhibitory peptide from casein and evaluation of the inhibitory mechanisms. Food Chem. 2018 Aug 1;256:98-104. doi: 10.1016/j.foodchem.2018.02.107. Epub 2018 Feb 21. PMID: 29606478.
  16. Gao B, Doan A, Hybertson BM. The clinical potential of influencing Nrf2 signaling in degenerative and immunological disorders. Clin Pharmacol. 2014 Feb 3;6:19-34. doi: 10.2147/CPAA.S35078. PMID: 24520207; PMCID: PMC3917919.
  17. Hu LF, Wang Y, Ren RJ. The antioxidant stress effect and regulatory mechanism of Keap1-Nrf2/ARE signaling pathway [J] International Journal of Pharmaceutical Research. 2016; 43(1): 146-152.
  18. Huerta C, Jiang X, Trevino I, Bender CF, Ferguson DA, Probst B, Swinger KK, Stoll VS, Thomas PJ, Dulubova I, Visnick M, Wigley WC. Characterization of novel small-molecule NRF2 activators: Structural and biochemical validation of stereospecific KEAP1 binding. Biochim Biophys Acta. 2016 Nov;1860(11 Pt A):2537-2552. doi: 10.1016/j.bbagen.2016.07.026. Epub 2016 Jul 27. PMID: 27474998.

Figures:

Similar Articles

Recently Viewed

  • The Femoral Head of Patients with Hip Dysplasia is not as Osteogenic as Iliac Crest Bone Location
    Philippe Hernigou*, Yasuhiro Homma, Arnaud Dubory, Jacques Pariat, Damien Potage, Charles Henri Flouzat Lachaniette, Nathalie Chevallier and Helene Rouard Philippe Hernigou*,Yasuhiro Homma,Arnaud Dubory,Jacques Pariat,Damien Potage,Charles Henri Flouzat Lachaniette,Nathalie Chevallier,Helene Rouard . The Femoral Head of Patients with Hip Dysplasia is not as Osteogenic as Iliac Crest Bone Location. J Stem Cell Ther Transplant. 2017: doi: 10.29328/journal.jsctt.1001001; 1: 001-007
  • The Potential Use of Dimethyltryptamine against Ischemia-reperfusion Injury of the Brain
    Attila Kovacs, Anna Mathe and Ede Frecska* Attila Kovacs, Anna Mathe, Ede Frecska*. The Potential Use of Dimethyltryptamine against Ischemia-reperfusion Injury of the Brain. J Neurosci Neurol Disord. 2024: doi: 10.29328/journal.jnnd.1001097; 8: 050-056
  • Incidental findings in traditional nuclear medicine practice
    Giuseppe De Vincentis, Mariano Pontico, Maria Ricci, Viviana Frantellizzi* and Mauro Liberatore Giuseppe De Vincentis,Mariano Pontico,Maria Ricci,Viviana Frantellizzi*,Mauro Liberatore. Incidental findings in traditional nuclear medicine practice. J Radiol Oncol. 2018: doi: 10.29328/journal.jro.1001019; 2: 036-040
  • Dapt Review
    KARACA Özkan*, KARASU Mehdi, KOBAT Mehmet A and KIVRAK Tarık KARACA Özkan*,KARASU Mehdi,KOBAT Mehmet A,KIVRAK Tarık. Dapt Review. J Cardiol Cardiovasc Med. 2020: doi: 10.29328/journal.jccm.1001088; 5: 060-066
  • Stem cells in heart failure some considerations
    Federico Benetti*, Natalia Scialacomo and Bruno Benetti Federico Benetti*,Natalia Scialacomo,Bruno Benetti. Stem cells in heart failure some considerations. J Stem Cell Ther Transplant. 2018: doi: 10.29328/journal.jsctt.1001009; 2: 001-003

Read More

Most Viewed

Read More

Help ?